5425R High-speed Frozen Centrifuge

Amen's 5425R high-speed frozen centrifuge (Centrifuge5425R), uhlobo lwenkinobho, uhlobo oluqandisiwe, rotor FA-24X2, 230V / 50-60Hz (CN), isixhumi esibonakalayo sokusebenza kwezinkinobho, ukuhlanzwa okulula. Ukubanda.

Amen's 5425R high-speed frozen centrifuge (Centrifuge5425R), uhlobo lwenkinobho, uhlobo oluqandisiwe, rotor FA-24X2, 230V / 50-60Hz (CN), isixhumi esibonakalayo sokusebenza kwezinkinobho, ukuhlanzwa okulula. Ukubanda. Njengomkhiqizo owalandela we-Centrifuge 5424 / 5424R ethandwayo, lawa mamodeli amabili amasha ahlanganisa yonke imikhiqizo oyithandayo amamodeli wangaphambilini, kanye nama-rotor amasha nezici zesofthiwe, okwenza umsebenzi wakho wansuku zonke usebenze kahle futhi ujabule kakhulu. Idizayini eyingqayizivele ye-Centrifuge5425 epholile emoyeni iqinisekisa ukusebenza okuthule ngokukhethekile ukuze kunikeze indawo yokusebenza enethezekile. Kuze kube yimizuzu eyi-12, i-centrifugation ingenziwa, futhi kusetshenziswe idizayini evimbela umsindo, futhi umsindo ojwayelekile ungu-45dB (a) kuphela. Le misebenzi yesikhathi esifushane yanele ukuqedela izinyathelo zokubeka i-centrifugation zamakhithi okuhlanza e-DNA/RNA ajwayelekile. Lapho isebenza isikhathi eside, ifeni yokupholisa ichithwa ngokuphumelelayo ngesikhathi sokusebenza okusele.

Izinhlelo zokupholisa ezithuthukisiwe ze-Centrifuge5425R ziqinisekisa umphumela omuhle wokuhlukanisa, ngisho nangesivinini esiphezulu se-rotor, ingapholiswa ngokuthembekile ku-4 ° C. Isikhathi esifushane nje esiyimizuzu engu-8 ngaphambi kokupholisa (~ 21 ° C kuya ku-4 ° C), i-centrifuge ingakwazi zisetshenzwe nganoma yisiphi isikhathi. Amarotha ahlukene manje amboza uhla olubanzi lwezinhlelo zokusebenza, okuhlanganisa ukusetshenziswa kwamangqamuzana ebhayoloji, okufana ne-DNA/RNA noma ukuhlanzwa kwamaprotheni, imvula yamagciwane, ekhuliswe kusukela ku-5 ml wamashubhu okuhlola, namaseli, kanye nokuhlolwa kwe-A trace, isibonelo, i-PCR pre. -ukwelashwa / ngemuva kokwelashwa kanye nohlelo lwe-QPCR lwakha ngeshubhu engu-0.2 ml, ipuleti elinemithombo engu-8, noma ipuleti elihlukaniswe izingxenye ezingu-96. I-Eppendorfkitrotor® (FA-18X2-Kit) isetshenziselwa amakholomu angu-18 x centrifugal kanye namashubhu angu-1.5 / 2.0ml centrifuge (ubukhulu obungu-18,565 xg). Le rotor eyingqayizivele ixazulula izinkinga ezivamile okuhlangatshezwana nazo uma usebenzisa ikhithi yekholomu ezungezayo ye-Miniprep: Ikhava yesiqukathi esivulekile siyanqanyulwa esinyathelweni sokulutha. Ngokuvamile kwenzekani le nkinga ukuthi ama-rotor endabuko awakwazi ukunikeza ukusekelwa okwanele kwama-caps we-centrifuge avuliwe ngesikhathi se-centrifugation. I-Eppendorfkitrotor inomphetho ophakanyisiwe obekwe kuwo ngesikhathi se-centrifugation, ngakho-ke ngeke anqunywe. Kusukela manje kuqhubeke, ikhava yakho yesiqukathi izohlala kusayithi layo. I-rotor FA-10×5 isetshenziswa kumashubhu angu-10 × 5.0mleppendorf centrifuge (afinyelela ku-21,300 xg), futhi amagciwane, imvubelo yesiko noma amaseli avunwa ku-Eppendorftubes® 5.0ml, kanye ne-DNA/RNA kanye ne-DNA plasmid purification isetshenziselwa ukuba elula kangaka. Ama-adaptha ahlinzekwa futhi ukuze kuzuzwe i-centrifugation kusetshenziswa amashubhu e-centrifuge angu-1.5 / 2 mL kanye namashubhu e-HPLC kanye ne-CRYO centrifugal ngaphandle kokushintsha i-rotor.

5425R izici ze-centrifuge eziqandisiwe ezinesivinini esikhulu:
1, ikhava ye-EppendorfquickLock® engenawo umoya, amasampula okuphatha alula kakhulu, aphephile;
2, izici ezinhle kakhulu zesoftware (ezifana nokusheshisa okulungisekayo kanye nemithambeka yebhuleki, izinkinobho zohlelo ezi-3, azikho izinqamuleli zezinkinobho eziqhubekayo);
3, ukuthuthukisa uhlelo lwe-rotor (isibonelo, i-rotor engu-5.0ml, ifanele izinga lepayipi le-PCR);
4, idizayini ye-OptiBowl®, qiniseka ukuthi ukusebenza okuthulile, noma ngabe ikhava yerotor nayo ithule kakhulu, yicindezele ngobumnene ukuze uvale ikhava ye-centrifugal, awekho amandla aqine kakhulu;
5, umthamo omkhulu we-rotor: 10 × 5.0ml centrifuge tube
6, amandla amakhulu e-centrifugal ahlobene: 21,300 × g (15,060 rpm)
7,5425R I-Freezing High Speed ​​​​Centrifuge ukusebenza komkhiqizo okuyingqayizivele, isistimu yokupholisa enhle kakhulu yokucubungula amasampula azwelayo okushisa angu-0.2-5 mL, ubuchwepheshe be-compressor obunelungelo lobunikazi, kunciphisa ukudlidliza, ukuvikela amasampuli. Ibanga lokushisa: -10 C kuya + 40 ° C.

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