CFX96TOUCH Fluorescent Quantitative PCR ithuluzi

I-CFX96Touch fluorescent quantitative PCR ingasetshenziswa emikhakheni eyahlukene yocwaningo ye-nucleic acid quantification, ukuhlaziywa kwezinga lesisho sofuzo, ukutholwa kokuguqulwa kofuzo, ukutholwa kwe-GMO, nokuhlaziywa okuqondile komkhiqizo.

Indawo yokusebenza:
1.1 Izinga lokushisa lokusebenza: 5-31 ° C
1.2 Ukusebenza nomswakama: umswakama ohlobene ≤80%
1.3 Amandla okusebenza: 100-240 VAC, 50-60Hz
Ukusebenza nezidingo zobuchwepheshe ze-CFX96Touch fluorescence quantitative PCR
3.1 Ukusebenza okuyinhloko (* inkomba okufanele ihlangatshezwe)
* 3.1.1 Iziteshi zokuhlola eziyisithupha, i-PCR engu-5% ingatholwa, futhi izakhi zofuzo ezihlosiwe ezi-5 zingatholwa ngesikhathi esifanayo, futhi isiteshi sokuthola i-FRET esikhethekile sitholwa kanyekanye.
* 3.1.2 Ngokusebenza kwe-PCR yezinga lokushisa eliguquguqukayo, ungagijima amazinga okushisa ayi-8 ahlukene ngesikhathi esisodwa, i-incubation ngayinye yezinga lokushisa.
3.1.3 Ukuvulwa kwama-reagents aphelele, ucwaningo oluhlukahlukene kanye nama-reagents omtholampilo ayasebenza;
3.1.4 Ifanele izinhlobonhlobo zezindlela ze-fluorescence ezifana ne-Taqman, i-Molecular Beacon, i-Fret probe, i-Sybr Green i, njll.;
3.1.5 Vula, ishubhu eyodwa engu-0.2ml, i-octal, ipuleti lemithombo engu-96, njll.
* 3.1.6 ingasebenza ngokuzimela, ukusebenza okungaxhunyiwe ku-inthanethi ngempela, asikho isidingo sokuxhuma ikhompuyutha ukuze igade ijika le-PCR le-fluorescent amplification ngesikhathi sangempela;
3.2 Izidingo zobuchwepheshe eziyinhloko (* inkomba okufanele ihlangatshezwe)
* 3.2.1 Umthamo wesampula: 96×0.2ml, ukucaciswa okujwayelekile kwamapuleti angama-96 (12×8) angasetshenziswa;
3.2.2 Uhlobo Lokuhlinzeka: 0.2ml ishubhu eyodwa, ukukhiya okuyisishiyagalombili, amapuleti angama-96, njll.
3.2.3 Uhlelo Lokuphendula: 1-50μL (kunconyiwe 10-25 μL);
* 3.2.4 Umthombo wokukhanya: Ama-LED ayisithupha anezihlungi;
* 3.2.5 Umtshina: Ama-diode ayisithupha e-photosensitive anezihlungi;
* Isivinini sokupholisa samalitha angu-3.2.6: 5 ° C / isekhondi;
3.2.7 Ibanga lokulawula izinga lokushisa: 0 -100 ° C;
3.2.8 Ukunemba Kwezinga Lokushisa: ± 0.2 ° C (90 ˚C);
3.2.9 Ukufana kwezinga lokushisa: ± 0.4 ° C (90 ˚C phakathi kwamasekhondi angu-10);
* 3.2.10 I-Dynamic Temperature Gradient Function: Sebenzisa amazinga okushisa angu-8 ahlukene ngesikhathi esisodwa; Ibanga lokulawula izinga lokushisa le-gradient: 30 -100 ° C; Ububanzi bomehluko wokushisa kwe-gradient: 1 - 24 ° C; isikhathi se-gradient incubation lokushisa: okufanayo;
3.2.11 i-excitation / emission wavelength range: 450-730 nm;
3.2.12 Ukuzwela: Kungatholwa isakhi sofuzo esiyikhophi eyodwa ku-genome yomuntu;
3.2.13 Ububanzi obunamandla: amanani ayi-10;
3.2.14 Isibonisi: 8.5 intshi umbala touch screen;
3.2.15 Imodi Yokuhlaziya Idatha: I-Standard Curve Quantity, Melting Curve, CT noma ΔΔCT Gene Expression Analysis, Multiple Internal Genodes Analysis and Amplification Efficiency Calculation, Multiple Data Files Gene Expression Analysis, Allelic Analysis, Type Analysis, Have Gene, umsebenzi wokuhlaziya ijika ;
3.2.16 Ukuthunyelwa Kwedatha: Excel, Word, noma PowerPoint. Umbiko womsebenzisi uqukethe izilungiselelo zokusebenzisa, ihluzo, nemiphumela yedatha yethebula, engaphrintwa noma ilondolozwe njenge-PDF;
* 3.2.17 Ucwaningo lwesakhiwo se-Chromosomal: Indlela yokuhlaziya ubuningi bezakhiwo ze-chromatin ngendima yokuqhathanisa yokuwohloka kwe-genomic DNA ngendima yokuqhathanisa ye-genomic DNA. Ifakazela ngempela ukuhlobana kobude phakathi kwesakhiwo se-chromatin nokuvezwa kofuzo;