Umgomo we-automatic nucleic acid extraction kanye nensimbi yokuhlanza

I-automatic nucleic acid extraction kanye nemishini yokuhlanza iwukukhipha nokuhlanza i-nucleic acid ngendlela yobuhlalu obuzibuthe, ngokuya ngokukhethwa kwekhithi ehambisanayo yamasampula emithombo eminingi (njengegazi, izicubu zezilwane nezitshalo, amaseli, njll.) ukuhlukaniswa kwe-nucleic acid. kanye nokuhlanzwa. Insimbi inokusebenza okuzinzile, umsindo ophansi, ukusebenza okuzenzakalelayo, okusheshayo nokulula, futhi ingahlanza amasampula angu-1-32 ngesikhathi esifanayo.
Umgomo wokusebenza:
Ngemuva kwesampula ye-lysis, ama-molecule e-nucleic acid akhishwe adsorbed ngokuqondile ebusweni bobuhlalu kazibuthe, futhi ama-nucleic acid ama-molecule ancibilika endaweni ngokumuncwa kazibuthe, ukudluliswa nokugeza ngezinduku kazibuthe ezakhelwe ngaphakathi. Ngezinhlobo ezahlukene zobuhlalu kazibuthe ama-nucleic acid reagents, i-nucleic acid emsulwa ingatholakala.
Imodi yokunyakaza kwebha kazibuthe: injini yokunyathela esetshenziswa emhlabeni wonke isetshenziswa njengethuluzi lokushayela, futhi umkhono webha kazibuthe ungalungisa ngokuzenzakalelayo i-amplitude yokudlidliza ngokwevolumu yesixazululo, ukuze isampula ixutshwe ngokugcwele; Isebenzisa idivayisi yokudlulisa isikulufu sebhola, umkhono wenduku kazibuthe kanye nenduku kazibuthe esebenza ngokuzinza, ukunemba okuphezulu, yelula impilo yesevisi; Ingxenye ngayinye ehambayo ifakwe umsebenzi wokuvikela isikhundla ukuze ugweme ukwehluleka ngempumelelo.
Imodi ye-adsorption enamandla: ngemodi yokukhangisa eqinile esanda kuklanywa, ubuhlalu kazibuthe bunamathiselwa ekhanda lenduku kazibuthe, ukuze kuqinisekiswe ukuthi ukukhanya kusengakwazi ukumboza bonke ubuhlalu kazibuthe ngisho noma ivolumu ye-lution incane kakhulu. Ubuhlalu obuzibuthe bunomphumela ongcono we-adsorption kanye nesivuno esiphezulu se-nucleic acid.

Izici ze-automatic nucleic acid extraction kanye nemishini yokuhlanza:
1, isibonisi sesikrini esithintwayo: 10.1 intshi yesikrini esikhulu esibonakalayo sombala we-Chinese, ukusebenza kokuthinta komuntu, ukwakheka kwenkinobho elula, kulula ukuyisebenzisa;
2, isampula design ihlukile: ipuleti isikhundla design womuntu, khulula ukuthatha izimpahla, ukugwema ukungcola kanye nezingozi ezibangelwa ukusebenza izithiyo;
I-3, umsebenzi wokuhlela onamandla: ungenza ngokwezifiso izinga lokushisa, uhlelo olusheshayo, ukhiye owodwa wokuqala, ungahlangabezana nezidingo zama-reagents ahlukene;
4, isizinda se-nucleic acid ngokushesha: isikhathi esifushane sokusebenza, i-15-39min ingaqedela ukukhishwa kwamasampula angama-32 (kuye ngokuthi i-reagent);
I-5, uhlelo lokusakaza ngezwi: abasebenzisi be-voice prompt bangalawula uhlelo ngokuya ngemodi yokulawula ejwayelekile, futhi batshele ukuqedwa kwemisebenzi yokuhlola;
6, izinga eliphezulu lokutakula: i-nucleic acid alulame & GT; 95%, ukubuyisela ubuhlalu kazibuthe & GT; 98%;
7, ukulawulwa kokungcoliswa kwemvelo: umklamo ovalwe ngokugcwele, isikhathi esakhelwe ngaphakathi sokubulala amagciwane e-UV, isiphephetha-moya esikhipha umoya, gwema ngempumelelo ukungcoliswa kwe-aerosol, i-alamu yokulwa nomnyango isebenza, qinisekisa ukuphepha kokusebenza;
8, isistimu yokusebenza ehlakaniphile: usebenzisa uhlelo lokusebenza lwe-Android, ungaphequlula ngokushesha Izilungiselelo zohlelo, slayida ukuze ukhethe izinhlelo, ushintshe izinhlelo zibe lula kakhulu.
Ukusetshenziswa nokugcinwa kwe-oven yokomisa
Ihhavini yokomisa ifanele ukuzwela okushisayo, ukubola kalula kanye nokomisa oxidative, isetshenziswa kabanzi kuma-elekthronikhi, amabhethri, insimbi, ipulasitiki, ukuxhumana, izimbotshana zamakhemikhali, izesekeli zezimoto nezithuthuthu, i-epoxy resin, izinto zokusetshenziswa zezimonyo, izinto zikazibuthe \ zamabhizinisi ezimboni. , amanyuvesi namakolishi, ucwaningo lwesayensi kanye nelabhorethri ehlobene yezinto, ukomisa, ukubhaka, ukuncibilika kwe-wax nokuvala inzalo.
Ukusetshenziswa kwe-oven yokumisa:
Umsebenzisi kufanele afunde imiyalelo ngokucophelela futhi ajwayelane nemisebenzi ye-incubator ngaphambi kokuvula amandla. Vula ukunikezwa kwamandla, cindezela inkinobho yamandla, inkomba yamandla ivuliwe. Lungisa isilawuli sezinga lokushisa kumazinga okushisa asethiwe adingwa umsebenzisi. Lapho izinga lokushisa lokubonisa le-incubator lifinyelela izinga lokushisa elibekiwe, ukushisa kuyaphazamiseka futhi inkomba yokushisa ivaliwe. Ngemuva kwemizuzu engu-90 yamandla evuliwe ezingeni lokushisa elijwayelekile le-ambient, izinga lokushisa lingahlala lizinzile. Uma izinga lokushisa elisheshayo ku-incubator lidlula izinga lokushisa eliphezulu le-alamu elimisiwe, inkomba ye-alamu yokulandelela izinga lokushisa yesilawuli sokushisa ivuliwe, futhi ukunikezwa kwamandla kwe-heater kunqanyulwa ngokuzenzakalelayo. Uma umnyango wengilazi uvulwa ukuthatha amasampula, i-heater kanye nomshini womoya ojikelezayo uzoyeka ukusebenza. Lapho umnyango wengilazi uvaliwe, i-heater kanye nefeni kungasebenza ngokujwayelekile, ukuze kugwenywe ukungcoliswa kwesiko kanye nokugcwala kwezinga lokushisa.
Ukugcinwa nokugcinwa kwe-oven yokomisa:
Gcina ingaphezulu le-incubator lihlanzekile futhi lihle. I-incubator kufanele ibekwe ekamelweni elinomoya omuhle, futhi izinto ezivuthayo neziqhuma akufanele zibekwe eduze kwayo. Ungafaki izinto ebhokisini eliminyene kakhulu, kufanele ushiye isikhala. Ingaphakathi nangaphandle kwebhokisi kufanele kugcinwe kuhlanzekile. Ngemuva kokusetshenziswa ngakunye, kufanele ihlanzwe. Uma ingasetshenzisiwe isikhathi eside, kufanele imbozwe ngesembozo sothuli futhi ifakwe ekamelweni elomile. Abasebenzi abaphethe izinto zokusebenza bazoqinisekisa i-metrological ngokohlelo lokuqinisekisa futhi bahlole ukulawulwa kwezinga lokushisa ngezikhathi ezithile. Uma izinga lokushisa lendawo lingaphansi kuka-40 ° C ehlobo, sebenzisa isipholile ukuze wehlise izinga lokushisa lendawo (25-28 ° C ebusuku) ukuze ugweme ukulahlekelwa kwezinga lokushisa. Ungabeki i-incubator endaweni eshisa kakhulu noma endaweni emanzi futhi ugweme ukukhanya kwelanga. Isiphephetha-moya esiku-incubator sihlala sigcwaliswa ngamafutha okugcoba. Uma ingasetshenziswa isikhathi eside, amanzi asebhantshini lamanzi kufanele akhululwe, futhi amafutha angathathi hlangothi noma i-vaseline kufanele afakwe ezingxenyeni ze-electroplated ukuvimbela ukugqwala. Isembozo sothuli lwepulasitiki kufanele sibekwe ngaphandle kwe-incubator, futhi i-incubator kufanele ibekwe ekamelweni elomile ukuze kugwenywe ukulimala okumanzi kwisilawuli sokushisa.
Izici zomkhiqizo:
1. I-studio ye-Cuboid, ekhulisa umthamo wokusetshenziswa.
I-2, idivayisi ekhethekile yokuqinisa imishini, ngokusetshenziswa kwe-liner yensimbi engagqwali eqinile, ukuqinisekisa ukusetshenziswa kwesikhathi eside kwe-deformation ye-liner yemishini.
3, umnyango wengilazi oqinile, ukuvalwa kahle, bheka izinto ezisendlini yokusebenza, shazi.
4, yonke indandatho yesivalo serabha ye-silicone, ukuqinisekisa ukusebenza okuhle kakhulu kokusebenza kwemishini.
5, ukulawulwa kwezinga lokushisa kusetshenziswa izinkinobho zokuthinta isibonisi sedijithali, isilungiselelo sokuthinta, isibonisi sedijithali nesiqondile, ukushisa kokulawula izinga lokushisa, ukupholisa, uhlelo oluzimele ngokuphelele lungathuthukisa ukusebenza kahle, lunciphise izindleko zokuhlola, lwandise ukuphila, lunciphise izinga lokuhluleka.
I-6, izinga lokushisa eliphelele lemishini; Isigaba semishini sisonke singaphansi/isigaba esiphambene; Ukugcwala kwemishini sekukonke; Isikhathi semishini sesisonke;
I-7, okunye ukuvuza, imiyalelo yokusebenza, ukuvala shaqa okuzenzakalelayo ngemva kokuvikelwa kwe-alamu yokwehluleka.


Isikhathi sokuthumela: Mar-13-2022