Ngokomgomo wokunqunywa kwe-nitrogen ye-Kjeldahl, izinyathelo ezintathu ziyadingeka ukuze kunqunywe, okuyi-digestion, i-distillation kanye ne-titration.
Ukugaya: Shisisa i-nitrogen-organic compounds (amaphrotheni) kanye ne-concentrated sulfuric acid kanye nama-catalysts (i-copper sulfate noma amaphilisi okugaya i-Kjeldahl) ukubola iphrotheni. Ikhabhoni ne-hydrogen kufakwa i-carbon dioxide namanzi ukuze abaleke, kuyilapho i-organic Nitrogen iguqulwa ibe i-ammonia (NH3) bese ihlanganiswa ne-sulfuric acid yakhe i-ammonium sulfate. (I-Ammonium NH4+)
Inqubo yokugaya ukudla: ukushisa ngokushisa okuphansi ukubilisa, into engaphakathi kwe-flask i-carbonized futhi ibe mnyama, futhi inqwaba yegwebu ikhiqizwa. Ngemuva kokuthi igwebu selinyamalele, khulisa amandla omlilo ukuze ugcine isimo sokubila kancane. Lapho uketshezi luba luhlaza okwesibhakabhaka-luhlaza futhi lucacile, qhubeka nokushisa 05-1h, futhi upholile ngemva kokuphela. (Ungasebenzisa ithuluzi lokugaya elizenzakalelayo ukuze uqedele umsebenzi wokucubungula ngaphambilini)
I-Distillation: Isixazululo esitholiwe sihlanjululwa sibe yivolumu engashintshi bese sengezwa nge-NaOH ukuze kukhululwe i-NH3 nge-distillation. Ngemuva kwe-condensation, iqoqwe kwisisombululo se-boric acid.
Inqubo ye-distillation: Okokuqala, isampula egayiwe iyahlanjululwa, i-NaOH iyengezwa, futhi igesi ye-ammonia ekhiqizwa ngemva kokushisisa ingena ku-condenser, futhi igeleza ebhodleleni elitholayo eliqukethe isixazululo se-boric acid ngemva kokufihlwa. Yakha i-ammonium borate. (Isikhombi esixubile siyengezwa esixazululweni se-boric acid. Ngemva kokwakhiwa kwe-ammonium borate, isixazululo esimuncayo siyashintsha sisuka ekubeni ne-asidi siye ku-alkaline, futhi umbala uyashintsha usuka kokunsomi uye koluhlaza okwesibhakabhaka.)
I-Titration: Titrate ngesisombululo esijwayelekile se-hydrochloric acid sokugxila okwaziwayo, bala okuqukethwe kwe-nitrogen ngokwenani le-hydrochloric acid esetshenzisiwe, bese uyiphindaphinda ngesici sokuguqula esihambisanayo ukuze uthole okuqukethwe kwamaprotheni. (I-Titration ibhekisela endleleni yokuhlaziya ubuningi kanye nokusebenza kokuhlolwa kwamakhemikhali. Isebenzisa ukusabela kobuningi kwezixazululo ezimbili ukuze inqume okuqukethwe kwe-solute ethile. Ibonisa indawo yokugcina ye-titration ngokuya ngokushintsha kombala wenkomba, bese ibheka ngokubonakalayo ukusetshenziswa kwesixazululo esijwayelekile Ivolumu, ukubala kanye nemiphumela yokuhlaziya.)
Inqubo ye-Titration: Yehlisa isixazululo esijwayelekile se-hydrochloric acid kusixazululo se-ammonium borate ukuze ushintshe umbala wesixazululo ukusuka kokuluhlaza okwesibhakabhaka ukuya kobomvu ngokukhanyayo.
I-DRK-K616 ezenzakalelayo ye-Kjeldahl nitrogen analyzer iwukuhlaziya okuzenzakalelayo okuhlakaniphile kokunquma kokuqukethwe kwe-nitrogen ngokusekelwe endleleni ye-Kjeldahl. Ingasetshenziswa kabanzi ekucutshungulweni kokudla, ekukhiqizeni ukudla, ugwayi, ukufuywa kwezilwane, umanyolo wenhlabathi, ukuqapha imvelo, imithi, ezolimo, ucwaningo lwesayensi, ukufundisa, ukuqondiswa kwekhwalithi kanye neminye imikhakha yokuhlaziywa kwe-nitrogen namaprotheni ku-macro kanye ne-semi-micro. amasampula. Ingase futhi isetshenziselwe usawoti we-ammonium, Ukutholwa kwama-acids/alkali anamafutha aguquguqukayo, njll. Uma usebenzisa indlela ye-Kjeldahl ukuze unqume isampula, idinga ukudlula ezinqubweni ezintathu zokugaya, ukuhluza, kanye ne-titration. I-Distillation ne-titration yizinqubo zokulinganisa eziyinhloko ze-DRK-K616 Kjeldahl nitrogen analyzer. I-DRK-K616 yohlobo lwe-Kjeldahl nitrogen analyzer iyisistimu yokulinganisa i-nitrogen ye-distillation ezenzakalelayo ngokugcwele eklanywe ngokuya ngendlela yakudala yokunquma i-nitrogen ye-Kjeldahl; leli thuluzi linikeza ukunethezeka okukhulu kubahloli baselabhorethri ohlelweni lokunquma i-nitrogen-protein. , Futhi inezici zokusetshenziswa okuphephile nokuthembekile; ukusebenza okulula nokonga isikhathi. I-interface yenkhulumomphendvulwano yamaShayina yenza umsebenzisi asebenzise kalula, isikhombimsebenzisi sinobungane, futhi ulwazi olubonisiwe lucebile, ukuze umsebenzisi akwazi ukuqonda ngokushesha ukusetshenziswa kwethuluzi.
Isikhathi sokuthumela: Feb-25-2022
